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Nom : BASTIEN
Prénom : Patrick
Equipe : BioGEPPE
Grade : PU-PH
Adresse email : Cette adresse e-mail est protégée contre les robots spammeurs. Vous devez activer le JavaScript pour la visualiser.
Téléphone : (+33) 4 67 33 23 50
Implantation : CHU La Colombière
Appartenance : UM1

Publications :

El Safadi D, Cian A, Nourrisson C, Pereira B, Morelle C, Bastien P, Bellanger AP, Botterel F, Candolfi E, Desoubeaux G, Lachaud L, Morio F, Pomares C, Rabodonirina M, Wawrzyniak I, Delbac F, Gantois N, Certad G, Delhaes L, Poirier P, Viscogliosi E (2016) Prevalence, risk factors for infection and subtype distribution of the intestinal parasite Blastocystis sp from a large-scale multi-center study in France. BMC Infectious Diseases 16: 451 doi

Nath-Chowdhury M, Sangaralingam M, Bastien P, Ravel C, Pratlong F, Mendez J, Libman M, Ndao M (2016) Real-time PCR using FRET technology for old world cutaneous leishmaniasis species differentiation. Parasites & Vectors 9: 255 doi

Ozbilgin A, Culha G, Uzun S, Harman M, Topal SG, Okudan F, Zeyrek F, Gunduz C, Ostan I, Karakus M, Toz S, Kurt O, Akyar I, Erat A, Gungor D, Kayabasi C, Cavus I, Bastien P, Pratlong F, Kocagoz T, Ozbel Y (2016) Leishmaniasis in Turkey: first clinical isolation of Leishmania major from 18 autochthonous cases of cutaneous leishmaniasis in four geographical regions. Tropical Medicine & International Health 21: 783-791 doi

Pratlong F, Balard Y, Lami P, Talignani L, Ravel C, Dereure J, Lefebvre M, Serres G, Bastien P, Dedet JP (2016) The Montpellier Leishmania collection, from a laboratory collection to a biological resource center: a 39-year-long story. Biopreservation and Biobanking 14: 470-479 doi

Stanojcic S, Sollelis L, Kuk N, Crobu L, Balard Y, Schwob E, Bastien P, Pagès M, Sterkers Y (2016) Single-molecule analysis of DNA replication reveals novel features in the divergent eukaryotes Leishmania and Trypanosoma brucei versus mammalian cells. Scientific Reports 6: 23142 doi

Tetaud E, Lefebvre M, M'Bang-Benet DE, Crobu L, Blancard C, Sterkers Y, Pagès M, Bastien P, Merlin G (2016) TbFlabarin, a flagellar protein of Trypanosoma brucei, highlights differences between Leishmania and Trypanosoma flagellar-targeting signals. Experimental Parasitology 166: 97-107 doi

Bastien P, Volf P, Depaquit J, Dondji B, Gallego M, Gangneux JP, Izri A, Marty P, Piarroux R, Pratlong F, Dedet JP (2015) Comments on Leishmania major in gorilla feces. Journal of Infectious Diseases 212: 505-U170 doi

Brenier-Pinchart MP, Capderou E, Bertini RL, Bailly S, Fricker-Hidalgo H, Varlet-Marie E, Murat JB, Sterkers Y, Touafek F, Bastien P, Pelloux H (2015) Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii. Diagnostic Microbiology and Infectious Disease 82: 289-291 doi

Casanova M, de Monbrison, F, van Dijk J, Janke C, Pagès M & Bastien P (2015) Characterisation of polyglutamylases in trypanosomatids. International Journal for Parasitology. 45: 121-132 doi

Filisetti D Yera H Villard O Escande B Wafo E Houfflin-Debarge V Delhaes L Bastien P (2015) Contribution of neonatal amniotic fluid testing to diagnosis of congenital Toxoplasmosis. Journal of Clinical Microbiology. 53: 1719-1721 doi

Filisetti D, Sterkers Y, Brenier-Pinchart M-P, Cassaing S, Dalle F, Delhaes L, Pelloux H, Touafek F, Varlet-Marie E, Yera H, Candolfi E & Bastien P (2015) Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis. Journal of Clinical Microbiology. 53: 29-34 doi

Mbang-Benet DE, Sterkers Y, Crobu L, Sarrazin A, Bastien P, Pagès M (2015) RNA interference screen reveals a high proportion of mitochondrial proteins essential for correct cell cycle progress in Trypanosoma brucei. BMC Genomics 16: 297 doi

Morelle C, Sterkers Y, Crobu L, Mbang-Benet DE, Kuk N, Portales P, Bastien P, Pagès M, Lachaud L (2015) The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids. Nucleic Acids Research 43: 4013-4027 doi

Robert-Gangneux F, Sterkers Y, Yera H, Accoceberry I, Menotti J, Cassaing S, Brenier-Pinchart MP, Hennequin C, Delhaes L, Bonhomme J, Villena I, Scherer E, Dalle F, Touafek F, Filisetti D, Varlet-Marie E, Pelloux H, Bastien P (2015) Molecular diagnosis of Toxoplasmosis in immunocompromised Patients: a 3-year multicenter retrospective study. Journal of Clinical Microbiology. 53: 1677-1684 doi

Sollelis L, Ghorbal M, MacPherson CR, Martins RM, Kuk N, Crobu L, Bastien P, Scherf A, Lopez-Rubio JJ, Sterkers Y (2015) First efficient CRISPR-Cas9-mediated genome editing in Leishmania parasites. Cellular Microbiology 17: 1405-1412 doi

Valdivia HO, Reis-Cunha JL, Rodrigues-Luiz GF, Baptista RP, Baldeviano GC, Gerbasi RV, Dobson DE, Pratlong F, Bastien P, Lescano AG, Beverley SM, Bartholomeu DC (2015) Comparative genomic analysis of Leishmania (viannia) peruviana and Leishmania (viannia) braziliensis. BMC Genomics 16: 715 doi

Cassagne C, Pratlong F, Jeddi F, Benikhlef R, Aoun K, Normand AC, Faraut F, Bastien P & Piarroux R (2014) Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clinical Microbiology and Infection. 20: 551-557 doi

Delhaes L, Filisetti D, Brenier-Pinchart M-P, Pelloux H, Yera H, Dalle F, Sterkers Y, Varlet-Marie E, Touafek F, Cassaing S & Bastien P (2014) Freezing and storage at -20 degrees C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis. Diagnostic Microbiology and Infectious Disease. 80: 197-199 doi

Fatoux-Ardore M, Peysselon F, Weiss A, Bastien P, Pratlong F & Ricard-Blum S (2014) Large-scale investigation of Leishmania interaction networks with host extracellular matrix by surface plasmon resonance imaging. Infection and Immunity. 82: 594-606 doi [- erratum -]

Lachaud L, Bourgeois N, Kuk N, Morelle C, Crobu L, Merlin G, Bastien P, Pagès M & Sterkers Y (2014) Constitutive mosaic aneuploidy is a unique genetic feature widespread in the Leishmania genus. Microbes and Infection. 16: 61-66 doi

Mbang-Benet DE, Sterkers Y, Morelle C, Kebe NM, Crobu L, Portales P, Coux O, Hernandez JF, Meghamla S, Pagès M & Bastien P (2014) The bacterial-like HslVU protease complex subunits are involved in the control of different cell cycle events in trypanosomatids. Acta Tropica. 131: 22-31 doi

Pigott DM, Bhatt S, Golding N, Duda KA, Battle KE, Brady OJ, Messina JP, Balard Y, Bastien P, Pratlong F, Brownstein JS, Freifeld C, Mekaru SR, Gething PW, George DB, Myers MF, Reithinger R & Hay SI (2014) Global distribution maps of the Leishmaniases. Elife. 3: e02851 doi

Sterkers Y, Crobu L, Lachaud L, Pagès M & Bastien P (2014) Parasexuality and mosaic aneuploidy in Leishmania: alternative genetics. Trends in Parasitology. 30: 429-435 doi

Varlet-Marie E, Sterkers Y, Brenier-Pinchart M-P, Cassaing S, Dalle F, Delhaes L, Filisetti D, Pelloux H, Touafek F, Yera H & Bastien P (2014) Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays. Journal of Clinical Microbiology. 52: 3952-3959 doi

Lachaud L, Dedet JP, Marty P, Faraut F, Buffet P, Gangneux JP, Ravel C, Bastien P & the working group for the notification of human leishmanioses in France (2013) Surveillance of leishmaniases in France, 1999 to 2012. Eurosurveillance. 18: 38-44

Zangger H, Ronet C, Desponds C, Kuhlmann FM, Robinson J, Hartley MA, Prevel F, Castiglioni P, Pratlong F, Bastien P, Muller N, Parmentier L, Saravia NG, Beverley SM & Fasel N (2013) Detection of Leishmania RNA virus in Leishmania parasites. PLoS Neglected Tropical Diseases. 7: e2006 doi

Morelle C, Varlet-Marie E, Brenier-Pinchart MP, Cassaing S, Pelloux H, Bastien P & Sterkers Y (2012) Comparative assessment of a commercial kit and two laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis. Journal of Clinical Microbiology. 50: 3977-3982 doi

Sterkers Y, Lachaud L, Bourgeois N, Crobu L, Bastien P & Pagès M (2012) Novel insights into genome plasticity in Eukaryotes: mosaic aneuploidy in Leishmania. Molecular Microbiology. 86: 15-23 doi

Sterkers Y, Pratlong F, Albaba S, Loubersac J, Picot M-C, Pretet V, Issert E, Boulot P & Bastien P (2012) Novel interpretation of molecular diagnosis of congenital toxoplasmosis according to gestational age at the time of maternal infection. Journal of Clinical Microbiology. 50: 3944-3951 doi

Vase MO, Hellberg YK, Larsen CS, Petersen E, Schaumburg H, Bendix K, Ravel C, Bastien P, Christensen M & d'Amore F (2012) Development of splenic marginal zone lymphoma in a HIV-negative patient with visceral leishmaniasis. Acta Haematologica. 128: 20-22 doi

Bañuls AL, Bastien P, Pomares C, Arevalo J, Fisa R & Hide M (2011) Clinical pleiomorphism in human leishmaniases, with special mention of asymptomatic infection. Clinical Microbiology and Infection. 17: 1451-1461 doi

Bastien P (2011) Leishmaniases control: what part for development and what part for research? Clinical Microbiology and Infection. 17: 1449-1450 doi

Sterkers Y, Lachaud L, Crobu L, Bastien P & Pagès M (2011) FISH analysis reveals aneuploidy and continual generation of chromosomal mosaicism in Leishmania major. Cellular Microbiology. 13: 274-283 doi

Sterkers Y, Ribot J, Albaba S, Issert E, Bastien P & Pratlong F (2011) Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood. Diagnostic Microbiology and Infectious Disease. 71: 174-176 doi

Bourgeois N, Bastien P, Reynes J, Makinson A, Rouanet I & Lachaud L (2010) 'Active chronic visceral leishmaniasis' in HIV-1-infected patients demonstrated by biological and clinical long-term follow-up of 10 patients. HIV Medicine. 11: 670-673

Smirlis D, Duszenko M, Ruiz AJ, Scoulica E, Bastien P, Fasel N & Soteriadou K (2010) Targeting essential pathways in trypanosomatids gives insights into protozoan mechanisms of cell death. Parasites & Vectors. 3: 15

Sterkers Y, Varlet-Marie E, Cassaing S, Brenier-Pinchart MP, Brun S, Dalle F, Delhaes L, Filisetti D, Pelloux H, Yera H & Bastien P (2010) Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens. Journal of Clinical Microbiology. 48: 3216-3222

Sterkers Y, Varlet-Marie E, Marty P & Bastien P (2010) Diversity and evolution of methods and practices for the molecular diagnosis of congenital toxoplasmosis in France: a 4-year survey. Clinical Microbiology and Infection. 16: 1594-1602

Casanova M, Crobu L, Blaineau C, Bourgeois N, Bastien P & Pagès M (2009) Microtubule-severing proteins are involved in flagellar length control and mitosis in Trypanosomatids. Molecular Microbiology. 71: 1353-1370

Chargui N, Amro A, Haouas N, Schonian G, Babba H, Schmidt S, Ravel C, Lefebvre M, Bastien P, Chaker E, Aoun K, Zribi M & Kuhls K (2009) Population structure of Tunisian Leishmania infantum and evidence for the existence of hybrids and gene flow between genetically different populations. International Journal for Parasitology. 39: 801-811

Lachaud L, Bourgeois N, Plourde M, Leprohon P, Bastien P & Ouellette M (2009) Parasite susceptibility to Amphotericin B in failures of treatment for visceral leishmaniasis in patients coinfected with HIV Type 1 and Leishmania infantum. Clinical Infectious Diseases. 48: E16-E22

Marin C, Dollet M, Pagès M & Bastien P (2009) Large differences in the genome organization of different plant Trypanosomatid parasites (Phytomonas spp.) reveal wide evolutionary divergences between taxa. Infection, Genetics and Evolution. 9: 235-240

Yera H, Filisetti D, Bastien P, Ancelle T, Thulliez P & Delhaes L (2009) Multicenter comparative evaluation of five commercial methods for Toxoplasma DNA extraction from amniotic fluid. Journal of Clinical Microbiology. 47: 3881-3886

Bastien P, Procop GW & Reischl U (2008) Quantitative real-time PCR is not more sensitive than "conventional" PCR. Journal of Clinical Microbiology. 46: 1897-1900

Bourgeois N, Lachaud L, Reynes J, Rouanet I, Mahamat A & Bastien P (2008) Long-term monitoring of visceral leishmaniasis in patients with AIDS - Relapse risk factors, value of polymerase chain reaction, and potential impact on secondary prophylaxis. Journal of Acquired Immune Deficiency Syndromes. 48: 13-19

Casanova M, Portales P, Blaineau C, Crobu L, Bastien P & Pagès M (2008) Inhibition of active nuclear transport is an intrinsic trigger of programmed cell death in trypanosomatids. Cell Death and Differentiation. 15: 1910-1920

Marin C, Alberge B, Dollet M, Pagès M & Bastien P (2008) First complete chromosomal organization of a protozoan plant parasite (Phytomonas spp.). Genomics. 91: 88-93

Bastien P, Jumas-Bilak E, Varlet-Marie E & Marty P (2007) Three years of multi-laboratory external quality control for the molecular detection of Toxoplasma gondii in amniotic fluid in France. Clinical Microbiology and Infection. 13: 430-433

Blaineau C, Tessier M, Dubessay P, Tasse L, Crobu L, Pagès M & Bastien P (2007) A novel microtubule-depolymerising kinesin involved in length control of a eukaryotic flagellum. Current Biology. 17: 778-782

Puechberty J, Blaineau C, Meghamla S, Crobu C, Pagès M & Bastien P (2007) Compared genomics of the strand switch region of Leishmania chromosome 1 reveal a novel genus-specific gene and conserved structural features and sequence motifs. BMC Genomics. 8:57-67

Dubessay P, Blaineau C, Bastien P, Tasse L, Van Dijk J, Crobu C & Pagès M (2006) Cell cycle-dependent expression regulation by the proteasome pathway and characterization of the nuclear targeting signal of a Leishmania major Kin-13 kinesin. Molecular Microbiology. 59:1162

Basset D, Faraut F, Marty P, Dereure J, Rosenthal E, Mary C, Pratlong F, Lachaud L, Bastien P & Dedet JP (2005) Visceral leishmaniasis in organ transplant recipients: 11 new cases and a review of the literature. Microbes and Infection. 7: 1370-1375

Chargui N, Bastien P, Kallel K, Haouas N, Akrout FM, Masmoudi A, Zili J, Chaker E, Ben Othman AD, Azaiez R, Crobu L, Mezhoud H & Babba H (2005) Usefulness of PCR in the diagnosis of cutaneous leishmaniasis in Tunisia. Transactions of the Royal Society of Tropical Medicine and Hygiene. 99: 762-768

Chabbert E, Lachaud L, Crobu L & Bastien P (2004) Comparison of two widely used PCR primer systems for detection of Toxoplasma in amniotic fluid, blood, and tissues. Journal of Clinical Microbiology. 42: 1719-1722

Bastien P, Chabbert E & Lachaud L (2003) Contamination management of broad-range or specific PCR: Is there any difference? Journal of Clinical Microbiology. 41: 2272-2272

Lachaud L, Chabbert E, Dubessay P, Dereure J, Lamothe J, Dedet JP & Bastien P (2002) Value of two PCR methods for the diagnosis of canine visceral leishmaniasis and the detection of asymptomatic carriers. Parasitology. 125: 197-207

Lachaud L, Marchergui-Hammami S, Chabbert E, Dereure J, Dedet JP & Bastien P (2002) Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. Journal of Clinical Microbiology. 40: 210-215

Lachaud L, Chabbert E, Dubessay P, Reynes J, Lamothe J & Bastien P (2001) Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood. Journal of Clinical Microbiology. 39: 613-617

Lachaud L, Dereure J, Chabbert E, Reynes J, Mauboussin JM, Oziol E, Dedet JP & Bastien P (2000) Optimized PCR using patient blood samples for diagnosis and follow-up of visceral leishmaniasis, with special reference to AIDS patients. Journal of Clinical Microbiology. 38: 236-240