Surname : STERKERS
Name : Yvon
Team :
BioGEPPE
Grade : MCU / PH
Email address : This email address is being protected from spambots. You need JavaScript enabled to view it.
Telephone : (+33) 4 67 63 55 13
Locality : CHU La Colombière
Employer : UM
Publications :
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Brenier-Pinchart MP, Varlet-Marie E, Robert-Gangneux F, Filisetti D, Guitard J, Sterkers Y, Yera H, Pelloux H, Bastien P (2021) Impact of pre-analytic step duration on molecular diagnosis of toxoplasmosis for five types of biological samples. PLoS One 16: e0246802 doi |
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Pasquier G, Azoury V, Sasso M, Laroche L, Varlet-Marie E, Houze S, Lachaud L, Bastien P, Sterkers Y, Leveque MF (2020) Rapid diagnostic tests failing to detect infections by Plasmodium falciparum encoding pfhrp2 and pfhrp3 genes in a non-endemic setting. Malaria Journal 19: 179 doi |
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Yagoubat A, Corrales RM, Bastien P, Leveque MF, Sterkers Y (2020) Gene editing in trypanosomatids: tips and tricks in the CRISPR-Cas9 era. Trends in Parasitology 36: 745-760 doi |
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Yagoubat A, Crobu L, Berry L, Kuk N, Lefebvre M, Sarrazin A, Bastien P, Sterkers Y (2020) Universal highly efficient conditional knockout system in Leishmania, with a focus on untranscribed region preservation. Cellular Microbiology 22: e13159 doi |
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Coulombe P, Nassar J, Peiffer I, Stanojcic S, Sterkers Y, Delamarre A, Bocquet S, Mechali M (2019) The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nature Communications 10: 2426 doi |
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Lévêque MF, Chiffre D, Galtier C, Albaba S, Ravel C, Lachaud L, Iemmi A, Flori P, Sterkers Y (2019) Molecular diagnosis of toxoplasmosis at the onset of symptomatic primary infection: a straightforward alternative to serological examinations. International Journal of Infectious Diseases 79: 131-133 doi |
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Roux G, Ravel C, Varlet-Marie E, Jendrowiak R, Bastien P, Sterkers Y (2019) Inhibition of polymerase chain reaction: pathogen-specific controls are better than human gene amplification. PLoS One 14: e0219276 doi |
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van der Laan S, Lévêque MF, Marcellin G, Vezenkov L, Lannay Y, Dubra G, Bompard G, Ovejero S, Urbach S, Burgess A, Amblard M, Sterkers Y, Bastien P, Rogowski K (2019) Evolutionary divergence of enzymatic mechanisms for tubulin detyrosination. Cell Reports 29: 4159-4176 doi |
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Vergnes B, Gazanion E, Mariac C, Du Manoir M, Sollelis L, Lopez-Rubio JJ, Sterkers Y, Bañuls AL (2019) A single amino acid substitution (H451Y) in Leishmania calcium-dependent kinase SCAMK confers high tolerance and resistance to antimony. Journal of Antimicrobial Chemotherapy 74: 3231-3239 doi |
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Claessens A, Harris LM, Stanojcic S, Chappell L, Stanton A, Kuk N, Veneziano-Broccia P, Sterkers Y, Rayner JC, Merrick CJ (2018) RecQ helicases in the malaria parasite Plasmodium falciparum affect genome stability, gene expression patterns and DNA replication dynamics. PLoS Genetics 14: e1007490 doi |
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Roux G, Varlet-Marie E, Bastien P, Sterkers Y, French National Reference Centre for Toxoplasmosis Network (2018) Evolution of Toxoplasma-PCR methods and practices: a French national survey and proposal for technical guidelines. International Journal for Parasitology 48: 701-707 doi |
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Varlet-Marie E, Sterkers Y, Perrotte M, Bastien P, on behalf of the 'Molecular Biology' working group of the French National Reference Centre for Toxoplasmosis (2018) A new LAMP-based assay for the molecular diagnosis of toxoplasmosis: comparison with a proficient PCR assay. International Journal for Parasitology 48: 457-462 doi |
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Barja PP, Pescher P, Bussotti G, Dumetz F, Imamura H, Kedra D, Domagalska M, Chaumeau V, Himmelbauer H, Pagès M, Sterkers Y, Dujardin JC, Notredame C, Spath GF (2017) Haplotype selection as an adaptive mechanism in the protozoan pathogen Leishmania donovani. Nature Ecology & Evolution 1: 1961-1969 doi |
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Garcia-Silva MR, Sollelis L, MacPherson CR, Stanojcic S, Kuk N, Crobu L, Bringaud F, Bastien P, Page M, Scherf A, Sterkers Y (2017) Identification of the centromeres of Leishmania major: revealing the hidden pieces. EMBO Reports 18: 1968-1977 doi |
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Stanojcic S, Kuk N, Ullah I, Sterkers Y, Merrick CJ (2017) Single-molecule analysis reveals that DNA replication dynamics vary across the course of schizogony in the malaria parasite Plasmodium falciparum. Scientific Reports 7: 4003 doi |
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Stanojcic S, Sollelis L, Kuk N, Crobu L, Balard Y, Schwob E, Bastien P, Pagès M, Sterkers Y (2016) Single-molecule analysis of DNA replication reveals novel features in the divergent eukaryotes Leishmania and Trypanosoma brucei versus mammalian cells. Scientific Reports 6: 23142 doi |
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Tetaud E, Lefebvre M, M'Bang-Benet DE, Crobu L, Blancard C, Sterkers Y, Pagès M, Bastien P, Merlin G (2016) TbFlabarin, a flagellar protein of Trypanosoma brucei, highlights differences between Leishmania and Trypanosoma flagellar-targeting signals. Experimental Parasitology 166: 97-107 doi |
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Brenier-Pinchart MP, Capderou E, Bertini RL, Bailly S, Fricker-Hidalgo H, Varlet-Marie E, Murat JB, Sterkers Y, Touafek F, Bastien P, Pelloux H (2015) Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii. Diagnostic Microbiology and Infectious Disease 82: 289-291 doi |
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Filisetti D, Sterkers Y, Brenier-Pinchart M-P, Cassaing S, Dalle F, Delhaes L, Pelloux H, Touafek F, Varlet-Marie E, Yera H, Candolfi E, Bastien P (2015) Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis. Journal of Clinical Microbiology. 53: 29-34 doi |
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Mbang-Benet DE, Sterkers Y, Crobu L, Sarrazin A, Bastien P, Pagès M (2015) RNA interference screen reveals a high proportion of mitochondrial proteins essential for correct cell cycle progress in Trypanosoma brucei. BMC Genomics 16: 297 doi |
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Morelle C, Sterkers Y, Crobu L, Mbang-Benet DE, Kuk N, Portales P, Bastien P, Pagès M, Lachaud L (2015) The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids. Nucleic Acids Research 43: 4013-4027 doi |
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Robert-Gangneux F, Sterkers Y, Yera H, Accoceberry I, Menotti J, Cassaing S, Brenier-Pinchart MP, Hennequin C, Delhaes L, Bonhomme J, Villena I, Scherer E, Dalle F, Touafek F, Filisetti D, Varlet-Marie E, Pelloux H, Bastien P (2015) Molecular diagnosis of Toxoplasmosis in immunocompromised Patients: a 3-year multicenter retrospective study. Journal of Clinical Microbiology. 53: 1677-1684 doi
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Sollelis L, Ghorbal M, MacPherson CR, Martins RM, Kuk N, Crobu L, Bastien P, Scherf A, Lopez-Rubio JJ, Sterkers Y (2015) First efficient CRISPR-Cas9-mediated genome editing in Leishmania parasites. Cellular Microbiology 17: 1405-1412 doi |
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Delhaes L, Filisetti D, Brenier-Pinchart M-P, Pelloux H, Yera H, Dalle F, Sterkers Y, Varlet-Marie E, Touafek F, Cassaing S & Bastien P (2014) Freezing and storage at -20 degrees C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis. Diagnostic Microbiology and Infectious Disease. 80: 197-199 doi
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Lachaud L, Bourgeois N, Kuk N, Morelle C, Crobu L, Merlin G, Bastien P, Pagès M & Sterkers Y (2014) Constitutive mosaic aneuploidy is a unique genetic feature widespread in the Leishmania genus. Microbes and Infection. 16: 61-66 doi
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Mbang-Benet DE, Sterkers Y, Morelle C, Kebe NM, Crobu L, Portales P, Coux O, Hernandez JF, Meghamla S, Pagès M & Bastien P (2014) The bacterial-like HslVU protease complex subunits are involved in the control of different cell cycle events in trypanosomatids. Acta Tropica. 131: 22-31 doi
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Sterkers Y, Crobu L, Lachaud L, Pagès M & Bastien P (2014) Parasexuality and mosaic aneuploidy in Leishmania: alternative genetics. Trends in Parasitology. 30: 429-435 doi
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Varlet-Marie E, Sterkers Y, Brenier-Pinchart M-P, Cassaing S, Dalle F, Delhaes L, Filisetti D, Pelloux H, Touafek F, Yera H & Bastien P (2014) Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays. Journal of Clinical Microbiology. 52: 3952-3959 doi
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Jugant S, Pernin V, Vetromile F, Garrigue V, Delmas S, Szwarc I, Sterkers Y & Mourad G (2013) Toxoplasma infection, a rare but life-threatening complication after kidney transplantation: report of two cases. Nephrologie & Therapeutique. 9: 32-36 doi |
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Morelle C, Varlet-Marie E, Brenier-Pinchart MP, Cassaing S, Pelloux H, Bastien P & Sterkers Y (2012) Comparative assessment of a commercial kit and two laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis. Journal of Clinical Microbiology. 50: 3977-3982 doi |
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Sterkers Y, Lachaud L, Bourgeois N, Crobu L, Bastien P & Pagès M (2012) Novel insights into genome plasticity in Eukaryotes: mosaic aneuploidy in Leishmania. Molecular Microbiology. 86: 15-23 doi |
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Sterkers Y, Pratlong F, Albaba S, Loubersac J, Picot M-C, Pretet V, Issert E, Boulot P & Bastien P (2012) Novel interpretation of molecular diagnosis of congenital toxoplasmosis according to gestational age at the time of maternal infection. Journal of Clinical Microbiology. 50: 3944-3951 doi |
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Sterkers Y, Lachaud L, Crobu L, Bastien P & Pagès M (2011) FISH analysis reveals aneuploidy and continual generation of chromosomal mosaicism in Leishmania major. Cellular Microbiology. 13: 274-283 doi
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Sterkers Y, Ribot J, Albaba S, Issert E, Bastien P & Pratlong F (2011) Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood. Diagnostic Microbiology and Infectious Disease. 71: 174-176 doi
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Sterkers Y, Varlet-Marie E, Cassaing S, Brenier-Pinchart MP, Brun S, Dalle F, Delhaes L, Filisetti D, Pelloux H, Yera H & Bastien P (2010) Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens. Journal of Clinical Microbiology. 48: 3216-3222
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Sterkers Y, Varlet-Marie E, Marty P & Bastien P (2010) Diversity and evolution of methods and practices for the molecular diagnosis of congenital toxoplasmosis in France: a 4-year survey. Clinical Microbiology and Infection. 16: 1594-1602 |
