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Notre équipe s’intéresse à l’évolution et à la diversité des styles de vie des virus.

Les virus sont les parasites par excellence. Le cycle de vie viral alterne entre une entité inactive métaboliquement mais responsable de la transmission (le virion) et une entité métaboliquement active, responsable de la réplication, transcription, et traduction du génome, et de la génération de virions (le virocell). Un virus infecte un hôte cellulaire de façon que l’ensemble forme un virocell. Depuis les origines de la vie cellulaire, les interactions entre virus et hôtes ont généré une énorme diversité de styles de vie virale ainsi que des gradients dans les interactions hôte-parasite: infection aigue à chronique; latente ou silencieuse; de bénigne ou asymptomatique à létale; de légèrement à extrêmement productive. Les travaux de notre équipe ont pour but de comprendre l’origine et l’évolution de la diversité des styles de vie des virus, par des approches bio-informatiques et expérimentales.

Notre modèle d’étude sont les virus à ADN, notamment les papillomavirus. Les papillomavirus (PVs) font partie du microbiote de la peau saine chez tous les mammifères. Les PVs ancestraux infectaient déjà les ancêtres des vertébrés. La plupart des infections par PVs chez les humains et chez les animaux sont asymptomatiques: nous sommes en fait tous infectés dès l’enfance. Les PVs peuvent aussi induire des proliférations bénignes, telles que les verrues, transmises par contact simple ou par rapport sexuel. Mais les infections chroniques par une poignée de PVs à transmission sexuelle sont responsables de 5% des cancers chez les humains, notamment le cancer du col de l’utérus, mais aussi d’autres sites anogénitaux et de la gorge. Malgré des dizaines d’années de recherche, ils restent encore de nombreuses questions ouvertes concernant la connexion évolutive entre les infections par PVs et les cancers.


Notre but ultime est de comprendre pourquoi seulement certains PVs sont associés aux cancers, tandis que la majorité des PVs causent des infections asymptomatiques.

 

virostyle.cnrs.fr

Membres de l'équipe

Publications 'MiVEGEC' par membres de l'équipe depuis 2005 :

| 2005 | | 2008 | | 2010 | | 2011 | | 2012 | | 2013 | | 2015 | | 2016 | | 2017 | | 2018 | | 2019 |

2019

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Alizon S, Bravo IG, Farrell PJ, Roberts S (2019) Towards a multi-level and a multi-disciplinary approach to DNA oncovirus virulence. Philosophical Transactions of the Royal Society B: Biological Sciences 374: 20190041 doi

Bedhomme S, Amoros-Moya D, Valero L, Bonifaci N, Pujana MA, Bravo IG (2019) Evolutionary changes after translational challenges imposed by horizontal gene transfer. Genome Biology and Evolution 11: 814-831 doi

Francois S, Mutuel D, Duncan AB, Rodrigues LR, Danzelle C, Lefèvre S, Santos I, Frayssinet M, Fernandez E, Filloux D, Roumagnac P, Froissart R, Ogliastro M (2019) A new prevalent densovirus discovered in Acari. Insight from metagenomics in viral communities associated with two-spotted mite (Tetranychus urticae) populations. Viruses 11: 233 doi

Froissart R, Brives C, Breyton C, Le Marrec C (2019) "French phage network" annual conference 2018 fourth meeting report. Viruses 11: 470 doi

Houlihan CF, Baisley K, Bravo IG, Pavon MA, Changalucha J, Kapiga S, De Sanjose S, Ross DA, Hayes RJ, Watson-Jones D (2019) Human papillomavirus DNA detected in fingertip, oral and bathroom samples from unvaccinated adolescent girls in Tanzania. Sexually Transmitted Infections 95: 374-379 doi

Murall CL, Rahmoun M, Selinger C, Baldellou M, Bernat C, Bonneau M, Boue V, Buisson M, Christophe G, D'Auria G, De Taroni F, Foulongne V, Froissart R, Graf C, Grasset S, Groc S, Hirtz C, Jaussent A, Lajoie J, Lorcy F, Picot E, Picot MC, Ravel J, Reynes J, Rousset T, Seddiki A, Teirlinck M, Tribout V, Tuaillon E, Waterboer T, Jacobs N, Bravo IG, Segondy M, Boulle N, Alizon S (2019) Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study. BMJ Open 9: e025129 doi

Pimenoff VN, Tous S, Benavente Y, Alemany L, Quint W, Bosch FX, Bravo IG, de Sanjose S (2019) Distinct geographic clustering of oncogenic human papillomaviruses multiple infections in cervical cancers: results from a worldwide cross-sectional study. International Journal of Cancer 144: 2478-2488 doi

Willemsen A, Bravo IG (2019) Origin and evolution of papillomavirus (onco)genes and genomes. Philosophical Transactions of the Royal Society B: Biological Sciences 374: 20180303 doi

2018

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Bagdassarian E, Doceul V, Pellerin M, Demange A, Meyer L, Jouvenet N, Pavio N (2018) The amino-terminal region of hepatitis E virus ORF1 containing a methyltransferase (Met) and a papain-like cysteine protease (PCP) domain counteracts type I interferon response. Viruses 10: 726 doi

Cassan C, Diagne CA, Tatard C, Gauthier P, Dalecky A, Ba K, Kane M, Niang Y, Diallo M, Sow A, Brouat C, Bañuls AL (2018) Leishmania major and Trypanosoma lewisi infection in invasive and native rodents in Senegal. PLoS Neglected Tropical Diseases 12: e0006615 doi

Francois S, Filloux D, Frayssinet M, Roumagnac P, Martin DP, Ogliastro M, Froissart R (2018) Increase in taxonomic assignment efficiency of viral reads in metagenomic studies. Virus Research 244: 230-234 doi

Gallet R, Froissart R, Ravigne V (2018) Experimental demonstration of the impact of hard and soft selection regimes on polymorphism maintenance in spatially heterogeneous environments. Evolution 72: 1677-1688 doi   reprint

Happel AU, Barnabas SL, Froissart R, Passmore JS (2018) Weighing in on the risks and benefits of probiotic use in HIV-infected and immunocompromised populations. Beneficial Microbes 9: 239-246 doi

Mena M, Taberna M, Tous S, Marquez S, Clavero O, Quiros B, Lloveras B, Alejo M, Leon X, Quer M, Bague S, Mesia R, Nogues J, Goma M, Aguila A, Bonfill T, Blazquez C, Guix M, Hijano R, Torres M, Holzinger D, Pawlita M, Pavon MA, Bravo IG, de Sanjose S, Bosch FX, Alemany L (2018) Double positivity for HPV-DNA/p16(ink4a) is the biomarker with strongest diagnostic accuracy and prognostic value for human papillomavirus related oropharyngeal cancer patients. Oral Oncology 78: 137-144 doi

Mengual-Chuliá B, Wibbelt G, Gottschling M, Bravo IG (2018) Two novel, distantly related papillomaviruses isolated from healthy skin of the Timor deer (Rusa timorensis). Microbiology Resource Announcements 6: e00505-18 doi

Mengual-Chuliá B, Wittstatt U, Olias P, Bravo IG (2018) Genome sequences of two novel papillomaviruses isolated from healthy skin of Pudu puda and Cervus elaphus deer. Microbiology Resource Announcements 6: e00298-18 doi

Taberna M, Torres M, Alejo M, Mena M, Tous S, Marquez S, Pavon MA, Leon X, Garcia J, Guix M, Hijano R, Bonfill T, Aguila A, Lozano A, Mesia R, Alemany L, Bravo IG (2018) The use of HPV16-E5, EGFR, and pEGFR as prognostic biomarkers for oropharyngeal cancer patients. Frontiers in Oncology 8: 589 doi

Willemsen A, Carrasco JL, Elena SF, Zwart MP (2018) Going, going, gone: predicting the fate of genomic insertions in plant RNA viruses. Heredity 121: 499-509 doi

2017

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Alizon S, Murall CL, Bravo IG (2017) Why human papillomavirus acute infections matter. Viruses 9: 293 doi

Bedhomme S, Pantoja DP, Bravo IG (2017) Plasmid and clonal interference during post horizontal gene transfer evolution. Molecular Ecology 26: 1832-1847 doi

Happel AU, Jaumdally SZ, Pidwell T, Cornelius T, Jaspan HB, Froissart R, Barnabas SL, Passmore JAS (2017) Probiotics for vaginal health in South Africa: what is on retailers' shelves? BMC Womens Health 17: 7 doi

Murall CL, Abbate JL, Touzel MP, Allen-Vercoe E, Alizon S, Froissart R, McCann K (2017) Invasions of host-associated microbiome networks. In, Networks of Invasion: Empirical Evidence and Case Studies. Advances in Ecological Research. 57: 201-281 doi

Nicolás-Párraga S, Alemany L, de Sanjosé S, Bosch FX, Bravo IG, RIS HPV TT and HPV VVAP study groups (2017) Differential HPV16 variant distribution in squamous cell carcinoma, adenocarcinoma and adenosquamous cell carcinoma. International Journal of Cancer 140: 2092-2100 doi

Pimenoff VN, de Oliveira CM, Bravo IG (2017) Transmission between archaic and modern human ancestors during the evolution of the oncogenic human papillomavirus 16. Molecular Biology and Evolution 34: 4-19 doi

Pontremoli C, Forni D, Cagliani R, Pozzoli U, Riva S, Bravo IG, Clerici M, Sironi M (2017) Evolutionary analysis of old world arenaviruses reveals a major adaptive contribution of the viral polymerase. Molecular Ecology 26: 5173-5188 doi

Torres-Barcelo C, Kaltz O, Froissart R, Gandon S, Ginet N, Ansaldi M (2017) "French phage network"-second meeting report. Viruses 9: 87 doi

Willemsen A, Zwart MP, Ambros S, Carrasco JL, Elena SF (2017) 2b or not 2b: Experimental evolution of functional exogenous sequences in a plant RNA virus. Genome Biology and Evolution 9: 297-310 doi

Willemsen A, Zwart MP, Elena SF (2017) High virulence does not necessarily impede viral adaptation to a new host: a case study using a plant RNA virus. BMC Evolutionary Biology 17: 25 doi

Zhang ZL, Gu QY, Montero MD, Bravo IG, Marques-Bonet T, Haussinger D, Munk C (2017) Stably expressed APOBEC3H forms a barrier for cross-species transmission of simian immunodeficiency virus of chimpanzee to humans. PLoS Pathogens 13: e1006746 doi

2016

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Alten B, Maia C, Afonso MO, Campino L, Jimenez M, Gonzalez E, Molina R, Bañuls AL, Prudhomme J, Vergnes B, Toty C, Cassan C, Rahola N, Thierry M, Sereno D, Bongiorno G, Bianchi R, Khoury C, Tsirigotakis N, Dokianakis E, Antoniou M, Christodoulou V, Mazeris A, Karakus M, Ozbel Y, Arserim SK, Kasap OE, Gunay F, Oguz G, Kaynas S, Tsertsvadze N, Tskhvaradze L, Giorgobiani E, Gramiccia M, Volf P, Gradoni L (2016) Seasonal dynamics of phlebotomine sand fly species proven vectors of Mediterranean leishmaniasis caused by Leishmania infantum. PLoS Neglected Tropical Diseases 10: e0004458 doi

Cassan C, Dione MM, Dereure J, Diedhiou S, Bucheton B, Hide M, Kako C, Gaye O, Senghor M, Niang AA, Bañuls AL, Faye B (2016) First insights into the genetic diversity and origin of Leishmania infantum in Mont Rolland (Thiès region, Senegal). Microbes and Infection 18: 412-420 doi

Félez-Sánchez M, Vergara M, de Sanjosé S, Castellsague X, Alemany L, Bravo IG, Grp Vvapo Ris Hpv Tt Study (2016) Searching beyond the usual papillomavirus suspects in squamous carcinomas of the vulva, penis and head and neck. Infection, Genetics and Evolution 45: 198-204 doi

François S, Filloux D, Roumagnac P, Bigot D, Gayral P, Martin DP, Froissart R, Ogliastro M (2016) Discovery of parvovirus-related sequences in an unexpected broad range of animals. Scientific Reports 6: 30880 doi

Mengual-Chulá B, Bedhomme S, Lafforgue G, Elena SF, Bravo IG (2016) Assessing parallel gene histories in viral genomes. BMC Evolutionary Biology 16: 32 doi

Nicolás-Párraga S, Gandini C, Pimenoff VN, Alemany L, de Sanjose S, Bosch FX, Bravo IG, Ris Hpv TT, Grp Hpv Vvap Study (2016) HPV16 variants distribution in invasive cancers of the cervix, vulva, vagina, penis, and anus. Cancer Medicine 5: 2909-2919 doi

Prudhomme J, Cassan C, Hide M, Toty C, Rahola N, Vergnes B, Dujardin JP, Alten B, Sereno D, Bañuls AL (2016) Ecology and morphological variations in wings of Phlebotomus ariasi (Diptera: Psychodidae) in the region of Roquedur (Gard, France): a geometric morphometrics approach. Parasites & Vectors 9: 578 doi

Senghor MW, Niang AA, Depaquit J, Ferte H, Faye MN, Elguero E, Gaye O, Alten B, Perktas U, Cassan C, Faye B, Bañuls AL (2016) Transmission of Leishmania infantum in the canine leishmaniasis focus of Mont-Rolland, Senegal: ecological, parasitological and molecular evidence for a possible role of Sergentomyia sand flies. PLoS Neglected Tropical Diseases 10: e0004940 doi

Willemsen A, Zwart MP, Higueras P, Sardanyes J, Elena SF (2016) Predicting the stability of homologous gene duplications in a plant RNA virus. Genome Biology and Evolution 8: 3065-3082 doi

Zhang ZL, Gu QY, Vasudevan AAJ, Hain A, Kloke BP, Hasheminasab S, Mulnaes D, Sato K, Cichutek K, Haussinger D, Bravo IG, Smits SHJ, Gohlke H, Münk C (2016) Determinants of FIV and HIV Vif sensitivity of feline APOBEC3 restriction factors. Retrovirology 13: 46 doi

2015

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Prudhomme J, Rahola N, Toty C, Cassan C, Roiz D, Vergnes B, Thierry M, Rioux JA, Alten B, Sereno D, Bañuls AL (2015) Ecology and spatiotemporal dynamics of sandflies in the Mediterranean Languedoc region (Roquedur area, Gard, France). Parasites & Vectors 8: 642 doi

2013

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Berngruber TW, Froissart R, Choisy M & Gandon S (2013) Evolution of virulence in emerging epidemics. PLoS Pathogens. 9: e1003209 doi

Doumayrou J, Avellan A, Froissart R & Michalakis Y (2013) An experimental test of the transmission-virulence trade-off in a plant virus. Evolution. 67: 477-486 doi

Doumayrou J, Leblaye S, Froissart R & Michalakis Y (2013) Reduction of leaf area and symptom severity as proxies of disease-induced plant mortality: The example of the Cauliflower mosaic virus infecting two Brassicaceae hosts. Virus Research. 176: 91-100 doi

2012

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Multeau C, Froissart R, Perrin A, Castelli I, Casartelli M & Ogliastro M (2012) Four amino acids of an insect densovirus capsid determine midgut tropism and virulence. Journal of Virology. 86: 5937-5941 doi pdf

Vale PF, Choisy M, Froissart R, Sanjuan R & Gandon S (2012) The distribution of mutational fitness effects of phage phi X174 on different hosts. Evolution. 66: 3495-3507 doi

2011

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Vuillaume F, Thebaud G, Urbino C, Forfert N, Granier M, Froissart R, Blanc S & Peterschmitt M (2011) Distribution of the phenotypic effects of random homologous recombination between two virus species. PLoS Pathogens. 7: e1002028 doi pdf

2010

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Elena SF & Froissart R (2010) New experimental and theoretical approaches towards the understanding of the emergence of viral infections Introduction. Philosophical Transactions of the Royal Society B: Biological Sciences. 365: 1867-1869 pdf

Froissart R, Doumayrou J, Vuillaume F, Alizon S & Michalakis Y (2010) The virulence-transmission trade-off in vector-borne plant viruses: a review of (non-)existing studies. Philosophical Transactions of the Royal Society B: Biological Sciences. 365: 1907-1918 pdf

2008

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Monsion B, Froissart R, Michalakis Y & Blanc S (2008) Large bottleneck size in Cauliflower Mosaic Virus populations during host plant colonization. PLoS Pathogens. 4: e1000174 pdf

2005

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Froissart R, Roze D, Uzest M, Galibert L, Blanc S & Michalakis Y (2005) Recombination every day: Abundant recombination in a virus during a single multi-cellular host infectiton. PLoS Biology. 3: e89 [- synopsis -] pdf

Montville R, Froissart R, Remold SK, Tenaillon O & Turner PE (2005) Evolution of mutational robustness in an RNA virus PLoS Biology. 3: e381 [- synopsis -] pdf

Plisson C, Uzest M, Drucker M, Froissart R, Dumas C, Conway J, Thomas D, Blanc S & Bron P (2005) Structure of the mature P3-virus particle complex of cauliflower mosaic virus revealed by cryo-electron microscopy Journal of Molecular Biology. 346: 267-277 pdf